I recently had problems when trying to access a webservice installed on a web site in IIS 6.0. When browsing to the web service at http://server/webservice.asmx I recieved: "Server Application Unavailable The web application you are attempting to access on this web server is currently unavailable. Please hit the "Refresh" button in your web browser to retry your request." The application event log returned two errors: Event Type: Error Event Source: ASP.NET 2.0.50727.0 Event Category: None Event ID: 1088 Description: Failed to execute request because the App-Domain could not be created. Error: 0x80070005 Access is denied. Event Type: Error Event Source: ASP.NET 2.0.50727.0 Event Category: None Event ID: 1334 Description: Failed to initialize the AppDomain:/LM/W3SVC/2079486950/Root Exception: System.IO.FileLoadException Message: Could not load file or assembly 'System.Web, Version=2.0.0.0, Culture=neutral, PublicKeyToken=b03f5f7f11d50a3a' or one of its dependencies. Access is denied. StackTrace: at System.Reflection.Assembly._nLoad(AssemblyName fileName, String codeBase, Evidence assemblySecurity, Assembly locationHint, StackCrawlMark& stackMark, Boolean throwOnFileNotFound, Boolean forIntrospection) at System.Reflection.Assembly.InternalLoad(AssemblyName assemblyRef, Evidence assemblySecurity, StackCrawlMark& stackMark, Boolean forIntrospection) at System.Reflection.Assembly.InternalLoad(String assemblyString, Evidence assemblySecurity, StackCrawlMark&...